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Published on: October 9, 2016
Promoter variant-dependent expression of the STAT5A gene in bovine liver
Krzysztof Flisikowski1, Rafał R Starzyński, Lech Zwierzchowski
1Department of Molecular Biology, Institute of Genetics and Animal Breeding Polish Academy of Sciences, Postepu 1, Jastrzebiec, 05-552 Wólka Kosowska, Poland. kflisikowski@yahoo.co.uk
Abstract:
Using PCR-heteroduplex and sequencing we identified A/G substitution at position -488 in the promoter region of the bovine STAT5A gene. With reverse transcription-PCR (RT-PCR) and other means we showed that the STAT5A expression level in the liver was higher in cattle with AA than GG genotype. Electrophoretic mobility shift assay (EMSA) showed that the A-->G transition at position -488 increased the STAT5A gene promoter binding capacity for liver nuclear proteins [possibly hepatic nuclear factor (HNF)-3].
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