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Published on: July 22, 2011
Differentiation of the major Listeria monocytogenes serovars by multiplex PCR
Michel Doumith1, Carmen Buchrieser, Philippe Glaser
1Laboratoire des Listeria-Centre National de Référence des Listeria-World Health Organization Collaborating Center for Foodborne Listeriosis, Institut Pasteur, 25 Rue du Dr. Roux, 75724 Paris Cedex 15, France. mdoumith@pasteur.fr
Abstract:
A new multiplex PCR assay was developed to separate the four major Listeria monocytogenes serovars isolated from food and patients (1/2a, 1/2b, 1/2c, and 4b) into distinct groups. The PCR test, which constitutes a rapid and practical alternative to laborious classical serotyping, was successfully evaluated with 222 Listeria strains.
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