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Metalloproteases secreted by Actinobacillus suis
Erasmo Negrete-Abascal1, Sergio Vaca Pacheco, Gloria L Paniagua
1Carrera De Biología, Facultad de Estudios Superiores Iztacala, UNAM, Av. de los Barrios # 1, Los Reyes Iztacala, Tlalnepantla, Estado de México, 54090, México. enegrete@correo.iztacala.unam.mx
Abstract:
Actinobacillus suis secretes metalloproteases into its medium. These secreted proteins, when concentrated by precipitation with 70% (NH4)2SO4 or methanol, displayed proteolytic activity at >200 kDa molecular mass bands in 10% polyacrylamide gels copolymerized with bovine casein (1%). They showed activity in a broad pH range (from pH 5 to pH 10) and were inhibited by 20 mM EDTA or EGTA, but could be reactivated by calcium. They were found heat stable at 40 degrees C, 50 degrees C, 60 degrees C, and 70 degrees C, but their activity diminished at 80 degrees C or higher. They degraded pig and bovine IgG and cross-reacted with a polyclonal serum against a high molecular mass secreted protease from A. pleuropneumoniae. Extracellular proteases could play a role in diseases caused by A. suis.