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Related Experiment Videos

Elastic light scattering from single cells: orientational dynamics in optical trap.

Dakota Watson1, Norbert Hagen, Jonathan Diver

  • 1Genoptix, San Diego, California, USA.

Biophysical Journal
|August 10, 2004
PubMed
Summary

Researchers studied light scattering from cells in an optical trap, revealing complex time-dependent patterns due to cell orientation dynamics. This work may enhance label-free flow cytometry sensitivity.

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Area of Science:

  • Biophysics
  • Optical physics

Background:

  • Light scattering provides information about particle properties.
  • Optical traps are used to manipulate and study microscopic particles.
  • Understanding cell dynamics is crucial for biological and medical applications.

Purpose of the Study:

  • To investigate the time-dependent light-scattering diagrams (phase functions) of single living cells and beads.
  • To correlate observed scattering patterns with the orientational dynamics of cells within an optical trap.
  • To explore the implications for improving label-free flow cytometry.

Main Methods:

  • Utilizing an optical trap to suspend single living cells and reference beads.
  • Recording light-scattering intensity over a wide angular range (0.5-179.5 degrees) with high time resolution (30 ms).

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  • Employing an optical setup with an elliptical mirror and rotating aperture for precise measurements.

    • Main Results:

    • Demonstrated significant and complex time dependence in light-scattering diagrams from biological cells.
    • Attributed this time dependence to the orientational dynamics of cells within the optical trap.
    • Calculated the time-dependent optical radiation pressure force based on measured phase functions and particle orientation.

    Conclusions:

    • Cellular orientational dynamics significantly influence light-scattering patterns.
    • The study provides insights into the forces acting on trapped particles based on their orientation.
    • Findings suggest potential for enhanced sensitivity in label-free flow cytometry through analysis of dynamic light scattering.