Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Using sampling techniques in protein crystallization.

H S Shieh1, W C Stallings, A M Stevens

  • 1Searle/Monsanto, BB4K, Department of Medicinal and Structural Chemistry, North Chesterfield, MO 63198-0001, USA.

Acta Crystallographica. Section D, Biological Crystallography
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aberrantly low STAT3 and STAT5 responses are associated with poor outcome and an inflammatory gene expression signature in pediatric acute myeloid leukemia.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2021
Same author

Switch from BAK-preserved to preservative-free latanoprost decreases anterior chamber flare in POAG patients.

International ophthalmology·2017
Same author

Genetic association of CD247 (CD3ζ) with SLE in a large-scale multiethnic study.

Genes and immunity·2015
Same author

Assessing and ensuring patient safety during breath-holding for radiotherapy.

The British journal of radiology·2014
Same author

Independent contribution of bronchoalveolar lavage and serum galactomannan in the diagnosis of invasive pulmonary aspergillosis.

Transplant infectious disease : an official journal of the Transplantation Society·2014
Same author

Breast cancer risk following Hodgkin lymphoma radiotherapy in relation to menstrual and reproductive factors.

British journal of cancer·2013

Optimizing macromolecule crystallization involves many factors. A mathematical sampling technique, MON48, simplifies this multi-variable problem for successful protein crystallization.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Crystallography

Background:

  • Macromolecule crystallization is crucial for structural determination.
  • Numerous variables (precipitant, pH, ions, etc.) complicate finding optimal crystallization conditions.
  • Identifying the ideal parameter combination presents a multi-variable optimization challenge.

Purpose of the Study:

  • To present a mathematical approach for optimizing macromolecule crystallization.
  • To introduce a simplified sampling technique for protein crystallization.
  • To demonstrate the successful application of the MON48 procedure.

Main Methods:

  • Utilizing mathematical sampling techniques to address multi-variable optimization.
  • Implementing an iterative procedure involving random sampling.

Related Experiment Videos

  • Incorporating quantitative evaluation and weighted sampling cycles.
  • Applying the MON48 procedure for protein crystallization.

    • Main Results:

    • The MON48 procedure, based on sampling techniques, was successfully applied to various protein crystallization problems.
    • Demonstrated a systematic approach to navigate the complex parameter space of crystallization.
    • Provided a simplified yet effective method for optimizing crystallization conditions.

    Conclusions:

    • Mathematical sampling techniques offer a robust framework for macromolecule crystallization optimization.
    • The MON48 procedure provides a practical and effective solution for protein crystallization challenges.
    • This approach facilitates the determination of protein structures through improved crystallization outcomes.