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Published on: May 16, 2017
Crystallization and preliminary X-ray analysis of the river buffalo (Bubalus bubalis L.) BB phenotype
A Zagari1, L Savino, S Capasso
1Centro di Studio di Bioscristallografia, CNR, Dipartimento di Chimica, Napoli, Italy.
Abstract:
Ruminant haemoglobin (Hb) extracted from river buffalo (Bubalus bubalis) has been purified and crystallized. Two different Hb forms of the phenotype BB gave isomorphous crystals which diffracted to 2.8 A resolution and were not sensitive to radiation damage. Crystals of CO Hb have space group P2(1)2(1)2(1) with unit-cell parameters a = 54.8, b = 64.0, c = 158.6 A, and contain one Hb molecule per asymmetric unit.
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