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Updated: Aug 23, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Published on: July 9, 2021
High-resolution crystals and preliminary X-ray diffraction studies of a catalytic RNA
R Kim1, E L Holbrook, J Jancarik
1Department of Chemistry and Structural Biology Division of Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
Abstract:
High-resolution single crystals of a catalytic RNA molecule derived from the sequence of the satellite RNA of tobacco ringspot virus have been obtained. The unit-cell volumes of the RNA crystals vary depending on the crystallization conditions and temperature. The best crystal form, when flash frozen, has space group P1 with unit-cell dimensions a = 53.08, b = 71.81, c = 28.03 A, alpha = 98.43, beta = 104.32 and gamma = 74.54 degrees. This form diffracts to a resolution of 2.4 A. A heavy-atom derivative search is in progress.
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