Crystallization and preliminary X-ray analysis of a lipase from Chromobacterium viscosum

Lipase from Chromobacterium viscosum has been purified to homogeneity and crystallized in a form suitable for X-ray diffraction analysis from 10-14% polyethylene glycol 4000 and 10-14% 2-methyl-2,4-pentane diol at pH 6.4 in the presence of 0.25%(w/v) n-octyl-beta-D-glucopyranoside. These crystals belong to space group P2(1)2(1)2 with refined lattice constants a = 41.1 A, b= 156.8, c = 43.6 A, indicating a cell content of one monomer per asymmetric unit of the crystal. The crystals diffract to a resolution of 2.2 A.