Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phasing macromolecular structures via structure-invariant algebra.

H Hauptman1, F Han

  • 1Medical Foundation of Buffalo, Inc., New York 14203, USA.

Acta Crystallographica. Section D, Biological Crystallography
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Becotatug Vedotin for Recurrent/Metastatic Nasopharyngeal Carcinoma (Magic-M001): A Multicenter, Randomized Trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

[Intestinal involvement in systemic lupus erythematosus: a clinicopathological analysis of three cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

[Quantitative analysis of policy texts on occupational health technical service institutions in China based on policy tools].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2026
Same author

[Clinical efficacy of multidisciplinary team collaboration in the treatment of deep sternal wound infection].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2025
Same author

Measurement of the Positive Muon Anomalous Magnetic Moment to 127 ppb.

Physical review letters·2025
Same author

[Clinical efficacy of composite transplantation of fascia lata-anterolateral thigh flap in repairing complex defects after radical tumor resection in maxillofacial region].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2025
Same journal

Structural insights into the synthesis of FMN in prokaryotic organisms.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

The first crystal structure of the peptidase domain of the U32 peptidase family.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.

Acta crystallographica. Section D, Biological crystallography·2015
See all related articles

This study presents two methods for determining crystal structure phases using diffraction intensities, especially when anomalous scatterers are present. The second method, which minimizes phase invariant differences, offers superior accuracy for phase determination.

Area of Science:

  • Crystallography
  • Materials Science
  • X-ray Diffraction

Background:

  • Friedel's law breaks down with anomalous scatterers, complicating direct phase determination.
  • Accurate crystal structure phase determination is crucial for understanding material properties.

Purpose of the Study:

  • To develop and compare methods for determining unique three-phase structure invariants and individual phases from diffraction intensities.
  • To address the challenge of phase ambiguity (2pi ambiguity) in crystallographic data analysis.

Main Methods:

  • A least-squares approach to resolve the 2pi ambiguity in triplet phase estimates.
  • A minimization method based on the 'minimal principle' to determine individual phase values by minimizing differences between estimated and true phase invariants.

Related Experiment Videos

  • Utilizing measured diffraction intensities to derive structure invariants.

    • Main Results:

    • Both methods successfully improve upon initial triplet phase estimates.
    • The second method, minimizing phase invariant differences, demonstrates superior performance compared to the least-squares approach.
    • The second method requires initial estimates of individual phases in addition to triplet phase estimates.

    Conclusions:

    • Accurate determination of three-phase structure invariants from diffraction intensities is achievable, even with anomalous scatterers.
    • The 'minimal principle' based method offers a more robust approach to solving the phase problem in crystallography.
    • Further refinement of phase determination techniques is essential for advancing crystallographic analysis.