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Crystallization and preliminary X-ray diffraction study of aldehyde reductase from a red yeast, Sporobolomyces
M Kataoka1, K Yamamoto, S Shimizu
1Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.
Abstract:
Crystals of aldehyde reductase from a red yeast, Sporobolomyces salmonicolor, have been grown from an ammonium sulfate solution, pH 7.0, by means of the vapor-diffusion procedure. The crystals belong to the hexagonal system, space group P6(1)22 or its enantiomorph, P6(5)22, with unit-cell dimensions of a = 72.2 and c = 320 A. The X-ray diffraction patterns extend to at least 2 A resolution with the use of synchrotron radiation. The crystals are stable on exposure to X-rays and suitable for high-resolution X-ray structure determination.
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