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Published on: December 30, 2016
Crystallization and preliminary X-ray crystallographic studies of glutamic acid-specific proteinase from Bacillus
1Shionogi Research Laboratories, Shionogi and Co. Ltd., Osaka, Japan.
Abstract:
A glutamic acid specific proteinase from Bacillus licheniformis has been crystallized as a complex with the inhibitor Z-Leu-Glu-CH(2)Cl. Crystals were grown by the vapor-diffusion method using sodium formate as a precipitant. The crystals diffracted to about 2.0 A resolution and belonged to the trigonal space group P3(1)21 (P3(2)21) with unit-cell parameters a = b = 134.3, c = 109.7 A. A total of 26 964 independent reflections were obtained up to 2.2 A resolution, the merging R-factor being 0.05 for 42 614 measurements.
