Related Experiment Video
Updated: Aug 23, 2026
07:42
On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
Published on: March 11, 2022
Crystallization and preliminary X-ray studies of plastocyanin from Silene expressed in E. coli
1Department of Applied Chemistry, Faculty of Engineering, Osaka University, Suita, Japan.
Acta Crystallographica. Section D, Biological Crystallography
|January 1, 1997
Abstract:
Plastocyanin from Silene (SilPc) expressed in E. coli has been crystallized in a form suitable for X-ray diffraction analysis by a macroseeding method using ammonium sulfate as a precipitant in acetate buffer (pH = 5.5). These crystals belong to the trigonal space group P3(1)21 or P3(2)21 with lattice parameters a = b = 76.6, c = 65.5 A, indicating an asymmetric unit containing two plastocyanin molecules. The crystals diffracted to at least 2.0 A.
