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[The analysis of tincture for improvement of blood circulation]
Rūta Bernatoniene1, Jurga Bernatoniene, Kristina Ramanauskiene
1Department of Drug Technology and Pharmaceutical Management, Kaunas University of Medicine, A. Mickeviciaus 9, 44307 Kaunas, Lithuania.
Abstract:
The article describes qualitative and quantitative analysis of tincture for improvement of blood circulation. Flavonoids have been qualitatively determined by methods of thin-layer chromatography, high-pressure liquid chromatography and color reaction with magnesium powder and concentrated hydrochloric acid. Ferments were identified with ferric ammonium sulphate solution; glycosides--with dimethylaminobenzaldehyde solution in sulphur acid; saponin--with lead subacetate solution; reductive materials - with silver nitrate ammoniacal solution; albumen--with ninhydrin solution; and caffeic acid--by method of thin-layer chromatography. An optimal spectrophotometrical method for determining the quantity of quercetin was applied. The refractive index, relative density, loss of drying and ethanol concentration were determined according to European Pharmacopoeia Requirements.