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Related Experiment Videos

Circulating hematopoietic progenitors with T lineage potential.

Benjamin A Schwarz1, Avinash Bhandoola

  • 1Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6160, USA.

Nature Immunology
|August 10, 2004
PubMed
Summary

Hematopoietic stem cells in adult mouse blood were identified. Lineage negative, stem cell antigen 1, and c-Kit (LSK) cells are the primary circulating progenitors with T cell development potential.

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Area of Science:

  • Immunology
  • Hematopoiesis
  • Stem Cell Biology

Background:

  • The thymus, crucial for T cell maturation, is populated by hematopoietic progenitors circulating in the blood.
  • The specific identity and T lineage potential of these circulating progenitors in adult mice remain largely unknown.

Purpose of the Study:

  • To identify the hematopoietic progenitors in adult mouse blood that possess efficient T cell lineage potential.
  • To characterize the phenotype and functional capacity of these circulating progenitors.

Main Methods:

  • Analysis of adult mouse peripheral blood.
  • Flow cytometry and cell sorting to isolate specific progenitor populations, including Lineage negative, Stem Cell Antigen 1, and c-Kit (LSK) cells.
  • Assessment of T lineage potential of sorted cell populations.

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Main Results:

  • The study identified Lineage negative, Stem Cell Antigen 1, and c-Kit (LSK) cells as the sole progenitors in circulation with significant T cell potential.
  • The blood LSK population comprises hematopoietic stem cells and multipotent progenitors, including early lymphoid progenitors and CD62L(+) cells.
  • Common lymphoid progenitors were not detected in the circulation.

Conclusions:

  • Circulating LSK cells are the principal hematopoietic progenitors responsible for seeding the thymus and possess robust T cell lineage potential.
  • Common lymphoid progenitors are unlikely to be physiological precursors for T cell development in adult mice.