Interlaboratory comparison to evaluate a standardized calibration procedure for the headspace analysis of aromatic...

The determination of volatile organic compounds in blood by headspace gas chromatography is one of the central and long-established analytical techniques in occupational medical biomonitoring. Nevertheless, the relatively low success rate in intercomparison programs shows that the headspace technique is insufficiently standardized. A critical stage of the analytical procedure seems to be the preparation of calibration standards in biological matrices. As part of an extensive interlaboratory comparison by the Analyses of Hazardous Substances in Biological Materials working group of the DFG Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, three typical procedures were compared with one another using typical aromatic solvents as an example. The best correlations between the participating laboratories and the best results for the analyses of samples from interlaboratory comparisons were obtained when highly concentrated stock solutions of the aromatic compounds in ethanol were first diluted with physiological saline and then used for spiking horse blood in headspace vials. This procedure can be easily standardized and is therefore recommended by the Analyses of Hazardous Substances in Biological Materials working group for the preparation of headspace calibration standards for aromatic compounds.