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Updated: Aug 23, 2026
Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
[Detection of fetal SRY gene in maternal plasma by real-time fluorescence quantitative PCR]
Chen-chun Ren1, Wen-jing Wang, Guo-chuan Liu
1Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin, 300052 PR China. rccxqy@sina.com.cn
Objective:
To isolate fetal DNA from maternal plasma and examine its fetal origin.
Methods:
Fetal DNA in maternal plasma was isolated from 150 samples in the first trimester and mid-trimester of pregnancy, respectively. Real-time fluorescence quantitative polymerase chain reaction PCR (FQ-PCR) was used to determine sex-determining region Y (SRY) gene on Y chromosome.
Results:
Eighty-two women in the first trimester and 90 women in the mid-trimester carried male fetuses,70 and 90 samples of them were positive, respectively. The mean concentrations were (58.82+/-20.90) copies/ml and (152.08+/-62.61) copies/ml. The results of FQ-PCR were negative in the women who carried female fetuses.
Conclusion:
The results show that fetal SRY gene can be found at a time as early as 42 days of gestation in maternal plasma by the use of FQ-PCR. The number of fetal DNA increases with gestational age. The real-time FQ-PCR is of great value in the non-invasive prenatal diagnosis.
