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Related Experiment Videos

Ethanol tolerance in a genetically insensitive selected mouse line.

M Limm1, J C Crabbe

  • 1Research Service, VA Medical Center, Portland, Oregon 97201.

Alcoholism, Clinical and Experimental Research
|August 1, 1992
PubMed
Summary

Both genetically resistant (HOT) and susceptible (COLD) mice developed significant ethanol tolerance when exposed to chronic cold temperatures. This suggests functional demand plays a key role in tolerance development, regardless of initial susceptibility.

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Area of Science:

  • Pharmacology
  • Neuroscience
  • Animal Models

Background:

  • Genetic differences in mice (COLD vs. HOT) influence ethanol's acute hypothermic effects.
  • Previous studies indicated differential tolerance development between COLD and HOT mouse lines.
  • Chronic ethanol administration at cold temperatures imposes a significant physiological stress.

Purpose of the Study:

  • To investigate ethanol tolerance development in genetically distinct mouse lines under chronic cold stress.
  • To determine if functional demand influences tolerance acquisition in ethanol-exposed mice.
  • To compare tolerance levels between COLD and HOT mouse lines under challenging environmental conditions.

Main Methods:

  • Chronic administration of ethanol to COLD and HOT mice at a cold ambient temperature (4°C).

Related Experiment Videos

  • Monitoring of hypothermic responses to assess acute ethanol effects.
  • Evaluation of tolerance development over repeated daily injections.
  • Dose-dependency analysis of ethanol tolerance.

    • Main Results:

    • Both COLD and HOT mouse lines exhibited significant ethanol tolerance by the third daily injection.
    • Initial hypothermic responses reached up to 10°C.
    • Tolerance development was dose-dependent, observed primarily at higher ethanol doses.
    • COLD and HOT mouse lines developed comparable degrees of tolerance.

    Conclusions:

    • Functional demand is a critical factor in the development of ethanol tolerance.
    • Environmental stressors, like cold, can induce tolerance even in genetically resistant lines.
    • Ethanol tolerance acquisition is not solely dependent on baseline susceptibility.