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DMBT1 expression is down-regulated in breast cancer
P Braidotti1, P G Nuciforo, J Mollenhauer
1University of Milano, Department of Medicine, Surgery and Dentistry, S. Paolo Hospital and IRCCS Ospedale Maggiore, Milan, Italy. paola.braidotti@unimi.it
BMC Cancer
|August 11, 2004
Summary
Deleted in malignant brain tumor 1 (DMBT1) is down-regulated in breast cancer, suggesting a role in tumor suppression. Its expression pattern also indicates a possible link to cell cycle regulation.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Investigated DMBT1 (deleted in malignant brain tumor 1), a potential tumor suppressor gene.
- Examined DMBT1 expression in normal, proliferative, and malignant breast tissues.
- Explored the relationship between DMBT1 and cell cycle regulation in breast epithelium.
Purpose of the Study:
- To analyze DMBT1 expression patterns in various breast tissue types.
- To determine if DMBT1 expression correlates with breast cancer development.
- To investigate the association between DMBT1 and cell proliferation markers.
Main Methods:
- Immunohistochemistry using anti-DMBT1 (DMBTh12) and anti-MCM5 antibodies on tissue sections.
- RT-PCR to assess DMBT1 mRNA levels in frozen tissue samples.
- Analysis of 17 benign lesions and 55 carcinomas.
Main Results:
- DMBT1 showed luminal polarized immunoreactivity in normal and hyperplastic epithelium.
- Loss of polarization and down-regulation of DMBT1 protein expression observed in cancerous lesions (p = 0.0001).
- RT-PCR confirmed immunohistochemical findings in 72% of cases; DMBT1 and MCM5 were co-expressed in most normal/hyperplastic cells.
Conclusions:
- Redistribution and down-regulation of DMBT1 in breast cancer suggest a tumor suppressor role.
- Concomitant expression of DMBT1 and MCM5 points to a potential association with cell-cycle regulation.
- Findings highlight DMBT1's potential significance in breast cancer pathogenesis and cell proliferation control.
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