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[Low temperature effects on sarcoplasmic reticulum membrane permeability for Ca2+].

G F Zhegunov, A M Belous

Ukrainskii Biokhimicheskii Zhurnal (1978)

|September 1, 1978

Summary

Rapid freezing to -196°C damages the sarcoplasmic reticulum (SR) Ca2+-pump. This impairs calcium transport and accumulation, affecting muscle function.

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Area of Science:

Biochemistry
Cell Biology
Membrane Physiology

Background:

Sarcoplasmic reticulum (SR) is crucial for muscle contraction by regulating intracellular calcium.
The SR Ca2+-pump (SERCA) actively transports calcium ions, maintaining low cytosolic calcium levels.
Understanding the impact of cryopreservation on SR function is vital for biological sample preservation.

Purpose of the Study:

To investigate the effects of rapid freezing at -196°C on the functional activity of the sarcoplasmic reticulum (SR) Ca2+-pump.
To determine how low temperatures impact calcium ion transport and ATPase activity within SR membranes.

Main Methods:

Rapid freezing of sarcoplasmic reticulum (SR) membrane vesicles to -196°C.
Assay of Ca2+-pump activity, including calcium ion transport and ATPase activity.
Measurement of calcium ion accumulation rates and Ca:ATP ratio in SR vesicles.

Main Results:

Rapid freezing significantly damages the SR Ca2+-transport system.
Low temperatures reduce the transport activity of Ca ions across the SR membrane.
ATPase activity slightly increases, while Ca ion accumulation rate and Ca:ATP ratio decrease.

Conclusions:

Cryopreservation via rapid freezing impairs the functional integrity of the SR Ca2+-pump.
Damage is characterized by increased Ca2+ leak and passive diffusion, alongside altered transport and ATPase activities.
These findings highlight the detrimental effects of freezing on SR membrane systems, impacting their calcium-handling capabilities.

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