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The 10 C-terminal residues of HTLV-I protease are not necessary for enzymatic activity
Bryan E Herger1, Victoria L Mariani, KellyJ Dennison
1Georgia Institute of Technology, School of Chemistry and Biochemistry, Atlanta 30332-0400, USA.
Abstract:
Sequence alignment of human T-lymphotropic virus type I (HTLV-I) protease and other retroviral proteases reveals that the leukemia virus proteases contain residues at the C-terminus that are absent in the other proteases. We have prepared a mutant of HTLV-I protease that does not contain the 10 C-terminal residues and demonstrated that the catalytic efficiency of cleavage of a peptide substrate is unaffected.
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