Related Experiment Video
Updated: Aug 23, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Lignin peroxidase production by Streptomyces viridosporus T7A: nitrogen nutrition optimization using glucose as
J E Zerbini1, E M Oliveira, E P Bon
1Departamento de Bioquímica, Instituto de Química, UFRJ, Rio de Janeiro, RJ, Brazil, 21949-900.
Abstract:
The production of lignin peroxidase by Streptomyces viridosporus T7A was studied in submerged batch fermentations using growth media containing 6.5 g/L yeast extract and 2.5-10.0 g/L glucose, corresponding to carbon to nitrogen (C/N) ratios from 7.1-12.4. The kinetics for biomass and enzyme accumulation and glucose consumption were followed allowing definition of optimized conditions for enzyme production. Considering the physiological response of the microorganism in relation to enzyme production, a sharp increase on enzyme activity was consistently observed upon glucose depletion, indicating glucose regulation. In accordance to that the plot of maximal enzyme vs maximal enzyme per gram of glucose consumption showed a linear inversely proportional relationship, indicating that the characteristics of the metabolic pool at the studied C/N ratios affected enzyme biosynthesis even after glucose depletion.
More Related Videos
Related Concept Videos
Production of Organic Acids
Production of Antibiotics
Bioreactor Controls-III
Production of Alcohol

