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Updated: Jul 24, 2026
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Published on: April 18, 2014
Expression of Trichoderma reesei exo-cellobiohydrolase I in transgenic tobacco leaves and calli
Z Dai1, B S Hooker, R D Quesenberry
1Bioprocessing Group, Environmental Technological Division, Pacific Northwest National Laboratory, P.O. Box 999, MSIN K2-10, Richland, WA 99352, USA. ziyudai@pnl.gov
Abstract:
Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 micromol/h/g total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 micromol h/g total protein. Protein immunoblot analysis confirms the presence of CBHI enzyme in both transgenic calli and leaf tissues. CBHI expression levels accounted for about 0.11% and 0.082% of total protein in transgenic leaf tissues and calli, respectively. Furthermore, expression of CBHI gene did not affect normal growth and development of transgenic plants.
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