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Updated: Aug 23, 2026

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Published on: July 20, 2016
The preparation of side chain functionalized analogues of coenzyme Q for protein conjugation studies
Alison M Daines1, Andrew D Abell
1Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
Abstract:
The synthesis of two analogues of CoQ (10 and 13) suitable for conjugation to a peptide or protein, and hence the development of an ELISA immunoassay, is presented. These analogues were synthesized from the protected quinone, 1-bromo-2-methyl-3,4,5,6-tetramethoxybenzene (1), itself prepared from commercially available CoQ-0 (3). Model coupling studies of one of the analogues (10) to N-acetyl-L-lysine methyl ester and a lysine containing dipeptide (N-acetyl-glycine-L-lysine methyl ester) were also undertaken as a first step to monoclonal antibody production.
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