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Related Experiment Videos

Interplay between cell division and cell death during TCR triggering.

Alexandre Boissonnas1, Behazine Combadiere

  • 1Faculté de Médecine Pitié-Salpétrière, Laboratoire d'Immunologie Cellulaire, INSERM U543, Paris, France.

European Journal of Immunology
|August 13, 2004
PubMed
Summary
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T cell expansion is regulated by cell death. T cells that divide once become susceptible to apoptosis, impacting immune responses and vaccine strategies.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • T cell expansion is critical for adaptive immunity.
  • Regulating T cell proliferation and death is essential for preventing autoimmunity and ensuring effective immune responses.
  • Antigen (Ag) dose influences T cell clonal amplification and elimination during primary responses.

Purpose of the Study:

  • To investigate the balance between T cell proliferation and death during T cell receptor (TCR) triggering.
  • To determine the susceptibility of T cells to cell death following primary stimulation with varying Ag doses.
  • To elucidate the timing and selectivity of apoptosis commitment during T cell activation.

Main Methods:

  • Utilized AND-TCR transgenic mice for CD4 T cell priming.
  • Administered varying doses of pigeon cytochrome c peptide 88-104 for primary stimulation.

Related Experiment Videos

  • Assessed T cell expansion, division, and cell death in vitro and in vivo.
  • Examined TCR re-engagement effects on activated T cells.

    • Main Results:

    • High antigen doses reduced T cell expansion rates due to increased clonal elimination, despite similar division numbers across doses.
    • TCR re-engagement induced cell death in activated T cells, regardless of the initial antigen dose.
    • Apoptosis commitment occurred as early as the first cell division in activated T cells.
    • Activated, non-dividing T cells were resistant to death, while cells dividing once became susceptible.

    Conclusions:

    • T cell death is a highly selective process, targeting cells after their first division.
    • These findings provide insights into peripheral immune homeostasis and antigen challenge.
    • The study has implications for optimizing primary and booster vaccine strategies by understanding T cell division-linked apoptosis.