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Related Experiment Videos

Surface effects on PCR reactions in multichip microfluidic platforms.

Nicholas J Panaro1, Xing Jian Lou, Paolo Fortina

  • 1Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Founders Pavilion, Philadelphia, PA 19104, USA.

Biomedical Microdevices
|August 17, 2004
PubMed
Summary

This study found common plastics, tubing, and syringes are compatible with polymerase chain reaction (PCR) microfluidic platforms. Adding PEG 8000 did not affect material compatibility for PCR applications.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Polymer Chemistry

Background:

  • Microfluidic platforms are increasingly used in biological and chemical analyses.
  • The selection of appropriate materials is critical for the success of microfluidic devices, especially for sensitive applications like polymerase chain reaction (PCR).
  • Commonly available plastics are attractive for low-cost microfluidic construction but their compatibility with PCR reagents needs thorough evaluation.

Purpose of the Study:

  • To assess the compatibility of various common plastics, plastic tubing, and disposable syringes with the polymerase chain reaction (PCR).
  • To evaluate the potential use of these materials in the construction of microfluidic platforms for PCR applications.
  • To investigate the influence of polyethylene glycol (PEG 8000) on material compatibility during PCR.

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Main Methods:

  • Construction of a simple, inexpensive plastic test module for material evaluation.
  • Compatibility testing of selected plastics, tubing, and syringes with PCR reaction mixtures.
  • Assessment of PCR performance in the presence of PEG 8000 and tested materials.

Main Results:

  • Several common plastics, commercially available plastic tubing, and disposable syringes demonstrated compatibility with the PCR reaction.
  • The addition of PEG 8000 to PCR reaction mixtures did not negatively impact the compatibility of the tested materials.
  • The developed test module proved effective for evaluating plastic materials for microfluidic PCR applications.

Conclusions:

  • Commonly available plastics, tubing, and syringes are suitable for constructing microfluidic platforms intended for PCR.
  • The use of PEG 8000 in PCR does not preclude the use of these compatible plastic materials.
  • These findings support the development of cost-effective microfluidic devices for molecular biology applications.