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[An improved animal model for chronic perforation of the tympanic membrane]
H Kaftan1, W Hosemann, A Beule
1Klinik und Poliklinik für Hals-Nasen-Ohren-Krankheiten, Kopf- und Halschirurgie der Ernst-Moritz-Arndt-Universität Greifswald.
Background:
An inexpensive and valid animal model of chronic tympanic membrane perforation is needed.
Method:
Twelve male rats were selected for different surgical procedures (subtotal tympanic membrane perforation with local microflaps, re-perforation without flaps, partial excision of the handle of malleus). The inhibition of spontaneous healing was accomplished by the application of prednisolon or mitomycin directly onto the tympanic membrane.
Results:
Only by additional partial excision of the handle of malleus followed by local application of mitomycin were we able to achieve a persistent tympanic membrane perforation.
Conclusion:
Chronic tympanic membrane perforation using this procedure may be useful in further investigations of the medical impact of tympanic membrane healing.
