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Changes in calcium uptake by liver induced by ferric lactate
L J Anghileri1, P Maleki, J Robert
1Biophysics Laboratory, Medicine Faculty, University of Nancy, France.
Archives of Biochemistry and Biophysics
|February 1, 1992
Summary
Ferric lactate enhances liver calcium uptake in vivo and in vitro. This effect is linked to ferric lactate binding to albumin, suggesting a cell membrane interaction rather than iron-induced cell damage.
Area of Science:
- Biochemistry
- Cell Biology
- Toxicology
Background:
- Ferric lactate is known to affect cellular processes.
- Calcium (Ca2+) homeostasis is crucial for liver function.
- Understanding iron compound interactions with cells is important for toxicology and medicine.
Purpose of the Study:
- To investigate the effect of ferric lactate on in vitro and in vivo calcium uptake by the liver.
- To elucidate the mechanism underlying ferric lactate-induced changes in calcium uptake.
Main Methods:
- In vitro and in vivo experiments measuring Ca(2+) uptake in liver tissue.
- Electrophoresis to assess ferric lactate binding to albumin.
- Use of deferoxamine as an inhibitor.
- Radioisotope (59Fe) tracking to quantify iron uptake.
Main Results:
- Ferric lactate significantly increased liver Ca(2+) uptake in vivo (2.9 nmol/g vs. 0.66 nmol/g in controls, P < 0.005).
- Albumin and deferoxamine inhibited these effects in vitro, and electrophoresis confirmed ferric lactate-albumin binding.
- The Ca(2+) uptake increase was reversible, while iron levels remained constant, indicating a non-damaging interaction.
Conclusions:
- Ferric lactate interacts with liver cell membranes, leading to increased Ca(2+) uptake.
- This interaction is likely mediated by binding to proteins like albumin.
- The findings support a cell membrane interaction mechanism over iron-catalyzed lipid peroxidation and cell injury.