Related Experiment Video
Updated: Aug 5, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
Published on: June 25, 2014
Amplification of genomic sequences flanking T-DNA insertions by thermal asymmetric interlaced polymerase chain
Yao-Guang Liu1, Yuanlin Chen, Qunyu Zhang
1South China Agricultural University, College of Life Science, Guangzhou, China.
Abstract:
Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) is an efficient tool for the recovery of DNA fragments adjacent to known sequences. A protocol is presented for the amplification of genomic sequences flanking DNA (T-DNA or transposon) insertions using the TAIL-PCR method. The amplified products are suitable as templates for direct sequencing, or for cloning in vectors. Examples are given in use of the protocol for T-DNA tagging in rice using the pCAMBIA binary vectors.
Related Concept Videos
PCR
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
PCR - Polymerase Chain Reaction
RACE - Rapid Amplification of cDNA Ends
Since the...
Sanger Sequencing
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

