Effect of in vitro Hemolysis on Values for Certain Porcine Serum Constituents

Serum consistuents were determined for 14 healthy pigs after the addition of hemolysates containing 54, 133, 215 and 400 mg/dl of hemoglobin to serum. Of sixteen chemical procedures considered, changes (P<0.0005) in creatinine, total protein, Pi, alanine aminotransferase (ALT), lactate dehydrogenase (LDH), Ca, K, aspartate aminotransferase (AST), total bilirubin and albumin values occurred following the addition of a hemolysate containing 400 mg/dl of hemoglobin. Changes (P<0.0005) were also observed in certain serum constituents when hemolysates containing 54, 133 and 215 mg/dl of hemoglobin were added.