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Updated: Aug 19, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Structural analysis of 22 full-length hepatitis B virus genomes isolated from patients with hepatocellular
1Research Center of Molecular Medicine, Fujian Medical University, Fuzhou 350004, China. Linxu70@hotmail.com
Objective:
To study the characteristics of full-length Hepatitis B Virus (HBV) genomes isolated from patients with hepatocellular carcinoma (HCC).
Methods:
The full-length HBV genomes from the serum of HBsAg positive HCC patients were amplified by PCR, and then sequenced and analyzed its structure.
Results:
Twenty-two full-length HBV DNAs were obtained from different patients of HCC. Phylogenetic analysis revealed that all HBV strains could be categorized into genotype B or C and serotype adr or adw2. Structural analysis showed that HBV obtained shared meaningful consensus mutations in B/T cell epitopes of surface and core antigens, transactivating domain of X protein and enhancer II/core promoter regions as compared to standard strains.
Conclusion:
Genotype and gene mutation of HBV may be closely correlated with the carcinogenesis of HBV-related hepatocellular carcinoma.
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