[Matrix metalloproteinase-9 expression by endothelial cells incubated with peripheral blood leukocytes from patients
1Department of Neurology, 1st Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Objective:
Matrix metalloproteinase-9 (MMP-9) have been implicatedin the development of cerebral infarction. In a prospective study we investigated whether the MMP-9 expression are increased by endothelial cells incubated with peripheral blood Leukocytes from patients with acute cerebral infarctions.
Methods:
Using Immuno-fluorescence technique, Human umbilical vein endothelial cells were cocultured with leucocytes for 24 hours in vitro before used for detecting the MMP-9 expression. The leucocytes were purified from peripheral blood in 6 patients with acute cerebral infarction and in 6 age- and sex-matched healthy subjects. the smears of endothelial cells were stained with MMP-9 monoclonal antibodies, and detected using the ABC ELITE kit.
Results:
Laser-Confocus confirmed the presence of MMP-9 within endothelial cells incubated with peripheral blood Leukocytes from patients with acute cerebral infarctions. Compared with the control group, MMP-9 expressions were significantly higher in the stroke group (P < 0.001).
Conclusion:
Our study demonstrated that the leukocytes from acute cerebral infarction patients were Activated, and could stimulate vessel endothelial cells excrete MMP-9. Activated leukocytes and MMP-9 expression by endothelial cells may have an important role in acute cerebrovascular ischemia and its consequences.
Insights
Leukocytes from acute cerebral infarction patients activate endothelial cells to express matrix metalloproteinase-9 (MMP-9). This MMP-9 expression may play a key role in cerebrovascular ischemia.
Area of Science:
- Biochemistry
- Immunology
- Vascular Biology
Context:
- Matrix metalloproteinase-9 (MMP-9) is implicated in cerebral infarction.
- Endothelial cells play a crucial role in vascular health and disease.
Purpose:
- To investigate if leukocytes from acute cerebral infarction patients increase MMP-9 expression in endothelial cells.
- To explore the role of activated leukocytes and MMP-9 in cerebrovascular ischemia.
Summary:
- Human umbilical vein endothelial cells were co-cultured with leukocytes from acute cerebral infarction patients and healthy controls.
- MMP-9 expression was detected using immunofluorescence and ABC ELITE kit.
- Significantly higher MMP-9 expression was observed in endothelial cells incubated with leukocytes from stroke patients compared to controls (P < 0.001).
Impact:
- Demonstrates that leukocytes from acute cerebral infarction patients are activated and stimulate endothelial MMP-9 excretion.
- Suggests activated leukocytes and endothelial MMP-9 expression are significant factors in acute cerebrovascular ischemia and its consequences.
