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[Liposomes mediated plasmids encoding human endostatin inhibits endothelial cell proliferation]
Wei Wang1, Xiao-guang Niu, Li-Xin Xie
1Shandong Eye Institute and Hospital, Qingdao 266071, China.
Objective:
To evaluate the effect of liposomes mediated plasmids encoding endostatin on endothelium cell proliferation.
Methods:
Cationic liposomes mediated endostatin (ES) expression of plasmid PCDNA(3)-ES was transferred to Cos-7 cells. ES protein expression was tested with immune dot blot. Human umbilical vein endothelial cells (ECV-304) was transfected by liposomes mediated PCDNA(3)-ES. The ES protein expression was tested with immunofluorescence staining. The effect of the transfection on cell proliferation was determined by methyl thiazolyl tetrazolium (MTT) method. The effect of ES on cell cycle after ES transfection was examined by flow cytometric analysis.
Result:
ES protein expression was detected in ES transfected COS-7 cells and supernatants. The ES expression in ECV-304 cells was reached the highest level on the second day after transfection. The proliferation of ECV-304 was inhibited by ES transfection with an increase in the ratio of G(0)/G(1) phase and a decrease in the ratio of the S phase.
Conclusion:
Liposomes mediated plasmids encoding ES can be transferred to endothelial cells and showed to inhibit proliferation of the endothelium cells.
