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Updated: Aug 23, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Nutritional deficiency affects cell cycle status and viability in A549 cells: role of p27Kip1
Takeo Ishii1, Mina Fujishiro, Michiaki Masuda
1Department of Pulmonary Medicine, Yokohama City University Medical Center, 4-57, Urahune-cho, Minami-ku, Yokohama City 232-0024, Japan.
Abstract:
We investigated how nutritional deficiency affects cell cycle and cell viability in A549 lung adenocarcinoma cells. Deprivation of various amino acids or glucose induced cell cycle arrest and cell death in a different manner. Cell death on deprivation of these nutrients was increased by downregulating of p27Kip1 with RNA interference. It was also observed that intrinsic p27Kip1 was segregated in cytoplasm in a glucose-deprived situation. In conclusion, amino acid or glucose deprivation induced cell cycle arrest and cell death, part of which is thought to be rescued by the existence of cytoplasmic p27Kip1.
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