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Nuclear pore complex oxalate binding protein p62: expression in different kidney disorders
P Sivakamasundari1, P Kalaiselvi, R Sakthivel
1Department of Medical Biochemistry, University of Madras, Taramani, Chennai-113, India.
Summary
Increased oxalate binding protein (p62) expression in kidney disease may indicate oxalate stress and serve as a diagnostic marker. This study also confirms p62 autoantibodies in hyperoxaluric patients.
Area of Science:
- Nephrology
- Molecular Biology
- Urology
Background:
- Urolithiasis involves microcrystal formation leading to kidney stones.
- Oxalate binding protein is crucial for oxalate transport.
- The role of oxalate binding protein in the nuclear pore complex is unclear.
Purpose of the Study:
- To investigate the role of oxalate binding protein (p62) in the nuclear pore complex.
- To assess p62 expression and autoantibodies in kidney disease patients.
Main Methods:
- Purified nuclear pore complex oxalate binding protein from human kidneys.
- Analyzed p62 protein expression and autoantibodies in patient urine and blood samples using ELISA.
- Compared patient data with healthy controls.
Main Results:
- Identified a 62 kDa nuclear pore complex oxalate binding protein.
- Hyperoxaluric patients showed threefold increased oxalate excretion and higher p62 expression.
- p62 autoantibodies were present in hyperoxaluria, SLE, and PBC patients.
Conclusions:
- Elevated p62 expression may result from oxalate-induced membrane damage and could be a diagnostic marker.
- Confirmed the presence of p62 autoantibodies in hyperoxaluric patients.