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[Establishment of a congenic strain with complement regulatory protein of factor H allotype derived from Mus.
S Natsuume-Sakai1, Y Harada, K Moriwaki
1Department of Immunobiology, Kanazawa University, Ishikawa, Japan.
Abstract:
Factor H is a plasma glycoprotein with M. W of 160 KDa which serves as one of the regulatory proteins for C 3 convertases. We have previously reported three serologically defined mouse factor H allotypes by surveying many laboratory and wild mice. In the present work, we established a congenic strain with factor H allotype, H. 2. on BALB/c (H. 1 allotype) background and named this strain BALB-H.2. Alloantiserum against each allotype has been easily prepared using two congenic strains by immunization with a small amount of whole mouse serum. BALB-H.2 is valuable for the genetic studies on the genes in the vicinity of factor H gene (cfh) derived from Mus. m. molossinus.
Insights
Researchers developed a new mouse model, BALB-H.2, to study the complement system
Area of Science:
- Immunology
- Genetics
Context:
- Factor H is a key regulator of the complement system, a crucial part of innate immunity.
- Previous work identified three mouse factor H allotypes.
Purpose:
- To establish a congenic mouse strain for studying the factor H gene (cfh).
Summary:
- A new congenic mouse strain, BALB-H.2, was created on a BALB/c background, possessing a distinct factor H allotype (H.2).
- This strain facilitates the easy preparation of alloantisera for factor H allotypes.
- The BALB-H.2 strain is valuable for genetic studies of the cfh gene and its surrounding regions.
Impact:
- Enables detailed genetic analysis of the factor H gene and its regulatory elements.
- Provides a valuable tool for understanding the complement system's regulation and associated diseases.