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ESTs, cDNA microarrays, and gene expression profiling: tools for dissecting plant physiology and development
Rob Alba1, Zhangjun Fei, Paxton Payton
1Boyce Thompson Institute for Plant Research, Cornell University Campus, Ithaca, NY, USA.
The Plant Journal : for Cell and Molecular Biology
|August 19, 2004
Summary
This study details gene expression profiling in plants using cDNA microarrays. It covers experimental design and data analysis for understanding plant development and comparative genomics.
Area of Science:
- Plant molecular biology
- Genomics
- Transcriptomics
Background:
- Gene expression profiling is crucial for understanding biological regulation.
- cDNA microarrays are a key technology for transcriptomic analysis in plants.
Purpose of the Study:
- To provide a comprehensive guide to gene expression profiling in plants using cDNA microarrays.
- To illustrate applications in plant physiology, development, and comparative genomics.
Main Methods:
- Fabrication of cDNA microarrays.
- Fluorescent labeling and cDNA hybridization techniques.
- Experimental design, data processing, and downstream analysis.
Main Results:
- Detailed methodologies for plant gene expression profiling are presented.
- Examples demonstrate applications in fruit development and ripening.
- Comparative transcriptome analysis between tomato and pepper fruit is discussed.
Conclusions:
- cDNA microarray technology offers valuable insights into plant gene expression.
- This approach facilitates understanding of plant physiology, development, and evolutionary genomics.