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Rapid, automated screening method for enzymatic transformations using a robotic system and supercritical fluid
Li Di1, Oliver J McConnell, Edward H Kerns
1Wyeth Research, P.O. Box CN 8000, Princeton, NJ 08543-8000, USA. dil@wyeth.com
Summary
A new automated method uses robotics and rapid chiral supercritical fluid chromatography (SFC) to speed up enzyme screening for biocatalysis. This accelerates the discovery of effective enzymes and reaction conditions for enzymatic transformations.
Area of Science:
- Biocatalysis and enzymatic transformations
- Analytical chemistry
- Process automation
Background:
- Enzyme screening is crucial for identifying biocatalysts for chemical synthesis.
- Current screening methods can be time-consuming, limiting the evaluation of enzyme libraries.
- Optimizing reaction conditions is essential for efficient enzymatic processes.
Purpose of the Study:
- To develop an automated screening method for enzymatic transformations.
- To accelerate the enzyme selection process for biocatalysis.
- To enable rapid evaluation of enzyme activity and enantioselectivity.
Main Methods:
- Development of an automated screening platform integrating a robotic system.
- Implementation of rapid chiral supercritical fluid chromatography (SFC) with 1.5-minute run times.
- Utilized kinetic resolution of secondary alcohols via enzymatic transesterification as a model system.
Main Results:
- Successfully developed and validated a rapid, automated screening method.
- Demonstrated significant acceleration of the enzyme screening workflow.
- The method effectively evaluates enzyme activity and enantioselectivity.
Conclusions:
- The developed automated method significantly enhances the efficiency of enzyme screening in biocatalysis.
- This approach facilitates faster identification of suitable biocatalysts.
- It is effective for both enzyme screening and reaction condition optimization.