Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA replication licensing affects cell proliferation or endoreplication in a cell type-specific manner.

María del Mar Castellano1, María Beatrice Boniotti, Elena Caro

  • 1Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.

The Plant Cell
|August 19, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Case Report: Epidemiologically inferred reverse zoonosis and intra-household zoonotic re-exposure of <i>Mycobacterium tuberculosis</i> in a domestic dog: a one health alert and the diagnostic potential of hypercalcaemia.

Frontiers in veterinary science·2026
Same author

A Study on Risk Factors for Bovine Tuberculosis in the Disease-Free Regions of Italy.

Pathogens (Basel, Switzerland)·2026
Same author

The HORMA-domain protein ASY1 recruits the plant-specific cyclin SDS to promote DMC1-mediated meiotic recombination.

The Plant cell·2026
Same author

Integrative sRNA, DNA Methylation, and Transcriptomics Reveals Dynamic Epigenetic Reprogramming of <i>Meloidogyne javanica</i>-Induced Galls in Arabidopsis.

International journal of molecular sciences·2026
Same author

A cytological framework of female meiosis in Arabidopsis.

The Plant cell·2026
Same author

Phosphate starvation induces root cell-type-specific transcriptional responses and alternative splicing.

The New phytologist·2026

DNA replication licensing factors CDC6 and CDT1 control cell division and development in Arabidopsis. Their regulation impacts cell proliferation and differentiation, maintaining developmental programs and organismal patterns.

Area of Science:

  • Plant molecular biology
  • Cell cycle regulation
  • Developmental biology

Background:

  • DNA replication licensing factors, such as CDC6 and CDT1, are essential for eukaryotic cell proliferation and genome stability.
  • The roles of these factors in whole organisms, particularly in relation to differentiation and development, are not well understood.

Purpose of the Study:

  • To investigate the regulation and function of Arabidopsis thaliana CDT1 (AtCDT1) in vivo.
  • To determine the consequences of altering DNA replication licensing control on plant development.

Main Methods:

  • Analysis of AtCDT1 transcriptional and post-transcriptional regulation.
  • Investigating the effects of manipulating CDC6 and CDT1 levels in developing Arabidopsis plants.

Related Experiment Videos

Main Results:

  • AtCDT1 availability is regulated transcriptionally by E2F and growth signals, and post-transcriptionally by CDK phosphorylation leading to proteasomal degradation.
  • Altered CDC6 or CDT1 levels result in cell type-specific effects: stimulated proliferation in leaf cells and extra endocycles in differentiating cells.

Conclusions:

  • CDC6 and CDT1 are critical targets for coordinating cell proliferation, differentiation, and development.
  • DNA replication licensing control is essential for maintaining proliferative potential, developmental programs, and morphogenetic patterns in plants.