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Streptococcus mutans genotypes isolated from root and coronal caries.

M M Nascimento1, J F Höfling, R B Gonçalves

  • 1Department of Oral Diagnosis, Faculty of Dentistry of Piracicaba, University of Campinas, Piracicaba, SP, Brazil.

Caries Research
|August 19, 2004
PubMed
Summary
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This study found that various Streptococcus mutans genotypes colonize oral cavities with caries. No specific genotype is uniquely linked to initiating or progressing coronal and root caries.

Area of Science:

  • Oral microbiology
  • Dental caries research
  • Molecular epidemiology

Background:

  • Streptococcus mutans is a primary etiological agent of dental caries.
  • Understanding the clonal diversity and distribution of S. mutans is crucial for caries prevention strategies.

Purpose of the Study:

  • To investigate the colonization patterns and clonal variations of Streptococcus mutans in individuals with coronal and root caries.
  • To determine if specific S. mutans genotypes are associated with different types of dental caries.

Main Methods:

  • Isolation and biochemical identification of mutans streptococci from saliva, plaque, and caries lesions.
  • Polymerase Chain Reaction (PCR) with specific primers for glucosyltransferase genes (gtfB, gtfI) to confirm species identity.
  • Arbitrary Primed PCR (AP-PCR) to assess genetic polymorphism and clonal distribution of S. mutans strains.

Related Experiment Videos

Main Results:

  • S. mutans was the most frequently isolated mutans streptococci (59.2%).
  • Each subject hosted multiple (2-10) S. mutans genotypes, distributed across different oral sites.
  • No significant difference in colonization variability was observed between carious and non-carious surfaces or between coronal and root caries.

Conclusions:

  • A wide spectrum of S. mutans clones can be present in individuals with dental caries.
  • No specific S. mutans genotype is uniquely responsible for the initiation or progression of either coronal or root caries.