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Localization of HstI transcripts to the apical ectodermal ridge in the mouse embryo
H R Suzuki1, H Sakamoto, T Yoshida
1Department of Biology, University of Iowa, Iowa City 52242.
Developmental Biology
|March 1, 1992
Summary
The HstI gene, a fibroblast growth factor family member, is found in developing mouse limbs. Its transcript localizes to the apical ectodermal ridge, suggesting a role in limb bud outgrowth during embryogenesis.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- The HstI gene encodes a protein belonging to the fibroblast growth factor family.
- Previous studies indicated a 3.0-kb transcript of the mouse HstI homolog in embryonic stages.
Purpose of the Study:
- To detect and localize HstI transcripts in developing mouse embryos.
- To investigate the potential role of HstI in limb bud development.
Main Methods:
- RNA hybridization and Polymerase Chain Reaction (PCR) were used to detect HstI transcripts.
- In situ hybridization was performed on serial embryo sections to localize transcripts.
- HstI cDNA was isolated for probe generation.
Main Results:
- A 3.0-kb HstI transcript was detected in Day 11 mouse embryos, specifically in limb and body regions.
- In situ hybridization revealed HstI transcript localization to the apical ectodermal ridge (AER) of fore- and hindlimb buds in Day 11 and 12 embryos.
- This localization occurred during the period of rapid limb bud distal growth.
Conclusions:
- HstI expression is restricted to the AER of developing limb buds.
- This specific localization suggests a crucial role for HstI in the normal embryogenesis of limb bud outgrowth.