Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus
J Cinatl1, G Hoever, B Morgenstern
1Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Paul-Ehrlich Str. 40, 60596, Frankfurt am Main, Germany. Cinatl@em.uni-frankfurt.de
Abstract:
To identify a model for the study of intestinal pathogenesis of severe acute respiratory syndrome (SARS) we tested the sensitivity of six human intestinal epithelial cell lines to infection with SARS coronavirus (SARS-CoV). In permissive cell lines, effects of SARS-CoV on cellular gene expression were analysed using high-density oligonucleotide arrays. Caco-2 and CL-14 cell lines were found to be highly permissive to SARS-CoV, due to the presence of angiotensin-converting enzyme 2 as a functional receptor. In both cell lines, SARS-CoV infection deregulated expression of cellular genes which may be important for the intestinal pathogenesis of SARS.
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