Analysis of synthetic peptides by capillary electrophoresis: effect of organic solvent modifiers and variable...

In this study, procedures based on volatile ammonium acetate buffer electrolytes of high pH value containing different organic solvent modifiers have been developed to achieve very high efficiency separations of histidine-containing synthetic peptides by high-performance capillary electrophoresis (HPCE) employing untreated fused silica capillaries. Different organic solvents, including acetonitrile, methanol and ethanol, at high volume fractions were used to modify the composition of the background buffer electrolyte. With the peptides investigated, it was found that methanol had the greatest effect in terms of enhancement of separation efficiency, as determined from the evaluation of theoretical plate numbers, N, of these HPCE systems. On the other hand, separation selectivities, e.g. the alpha(ij) values, did not change significantly as the volume fraction, psi, of the organic solvents was increased up to psi = 60% (v/v). Under these conditions, very rapid, e.g. 1-2 min, separation times could be still achieved. Compared to the effect of carrying out the separation of these peptides at constant voltage, a dramatic increase in the separation efficiency was also achieved by applying a linear voltage gradient during the HPCE experiment. Under optimal conditions of organic solvent composition and linear voltage gradient ramps, very high peak efficiencies for the studied set of synthetic peptides with N values of approximately 2-3 million theoretical plates per meter could be routinely obtained with fast analysis times. Moreover, these buffer electrolyte conditions are compatible with direct interfacing of the HPCE effluent to electrospray ionisation and ion trap mass spectrometers, thus expanding the analytical capabilities of these HPCE systems.