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Related Experiment Videos

Activity-dependent excitability changes in hippocampal CA3 cell Schaffer axons.

A F Soleng1, A Baginskas, P Andersen

  • 1Institute for Basic Medical Sciences, University of Oslo, Oslo, Norway.

The Journal of Physiology
|August 21, 2004
PubMed
Summary

Action potentials in axons cause brief excitability reduction followed by prolonged hyperexcitability. This increased excitability decay is influenced by membrane time constant and the hyperpolarization-activated current (Ih).

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Area of Science:

  • Neuroscience
  • Axonal Physiology
  • Computational Neuroscience

Background:

  • Membrane potential changes post-action potential in axons are crucial for synaptic function.
  • Direct intra-axonal recording is challenging, necessitating indirect methods like extracellular excitability testing.

Purpose of the Study:

  • To investigate the after-potentials following action potentials in unmyelinated cortical axons.
  • To understand the influence of temperature and ion concentrations on axonal excitability changes.
  • To explore the role of the hyperpolarization-activated current (Ih) in shaping these excitability dynamics.

Main Methods:

  • Extracellular excitability testing on CA3 soma in hippocampal slices.
  • Stimulating individual axonal branches to avoid local electrode artifacts.

Related Experiment Videos

  • Recording axonal action potentials using field recordings.
  • Modulating temperature and extracellular calcium (Ca2+) concentrations.
  • Utilizing the Ih blocker ZD7288.

    • Main Results:

    • A single action potential induced a 15 ms decrease in axonal excitability, followed by ~200 ms of increased excitability at 24°C.
    • Elevated temperature (34°C) reduced the initial depression but did not significantly alter the duration of hyperexcitability.
    • Increased stimulation rates (> 1 Hz) accelerated the decay of hyperexcitability, an effect partially mediated by Ih and blocked by ZD7288.

    Conclusions:

    • The decay of action potential-induced hyperexcitability is primarily determined by the membrane time constant.
    • The hyperpolarization-activated current (Ih) plays a role in modulating the decay of hyperexcitability at higher stimulation frequencies.
    • Extracellular excitability testing provides valuable insights into axonal after-potentials.