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In vitro propagation of emetic nut Randia dumetorum (Lamb.)
Ferdousi Begum1, Kazi Mohammed Didarul Islam, Rathindra Nath Paul
1Development of Biotechnology & Environmental Conservation Centre, House No 90, Road No 11/A, Dhanmondi R/A, Dhaka-1209, Bangladesh. debtec@aitlbd.net
Abstract:
An efficient protocol for in vitro shoot multiplication of Randia dumetorum (Emetic nut) has been developed. The seeds of R. dumetorum were germinated in vitro in MS medium in 5 weeks. Subsequent propagation using shoot tip as an explant was carried out in MS medium along with different concentrations and combinations of BAP (0.5-2.0) and NAA (0.0-2.0). Maximum shoot multiplication was obtained (12.7 shoots per shoot tip) in MS medium containing 1 mg/L BAP and 1 mg/L NAA. Micropropagated shoots were rooted in 1/2 MS medium supplemented with 1 mg/l IBA. This is the first report of in vitro plant propagation of R. dumetorum. In vitro grown plantlets showed a survival rate of 70% after 2 months of transplantation to natural environment.
