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Microplate screening assay for the detection of arsenite-oxidizing and arsenate-reducing bacteria
Diliana D Simeonova1, Didier Lièvremont, Florence Lagarde
1Laboratoire de Dynamique, Evolution et Expression de Génomes de Microorganismes, FRE 2326 CNRS -- Université Louis -- Pasteur, 28 rue Goethe, 67083 Strasbourg Cédex, France.
Abstract:
An efficient, inexpensive microplate colorimetric assay for screening of bacteria which can be used in bioremediation of arsenic was developed. The assay is based on the colorimetric analysis of the precipitates formed upon reaction of silver nitrate with arsenic. The method proved reliable and sensitive for the detection of As[III] oxidizers and As[V] reducers and can be used over a large pH range (5.8-8.4). Seventy-eight bacterial strains isolated from different environments were tested by this method. It also showed agreement with results obtained by high-performance liquid chromatography coupled with inductively coupled plasma atomic emission spectrometry.
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