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Published on: April 29, 2020
Nisin-controlled expression of Norwalk virus VP60 protein in Lactobacillus casei
M Cruz Martín1, María Fernández, José M Martin-Alonso
1Instituto de Productos Lácteos de Asturias (CSIC), 33300 Villaviciosa, Asturias, Spain.
FEMS Microbiology Letters
|August 24, 2004
Abstract:
A food-grade strain with nisRK stably integrated into the genome, was constructed in order to implement the nisin-controlled expression system (NICE) in Lactobacillus casei ATCC393. Expression of beta-glucuronidase (gus) reporter gene was employed to optimize the system, which has been successfully used to produce the main antigenic protein from Norwalk virus, opening new perspectives for producing edible vaccines.
