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Stem cell factor and H2O2 induce GLUT1 translocation in M07e cells
Tullia Maraldi1, Diana Fiorentini, Cecilia Prata
1Dipartimento di Biochimica G. Moruzzi, Università di Bologna, Via Irnerio 48, I-40126 Bologna, Italy.
Biofactors (Oxford, England)
|August 24, 2004
Summary
Stem cell factor (SCF) and hydrogen peroxide (H2O2) activate glucose transport in M07e cells by moving glucose transporter 1 (GLUT1) to the cell membrane. This process involves tyrosine kinases and phospholipase C (PLC).
Area of Science:
- Cell Biology
- Biochemistry
- Hematology
Background:
- Hematopoietic stem cell factor (SCF) is crucial for regulating blood cell development.
- Reactive oxygen species (ROS), such as hydrogen peroxide (H2O2), play complex roles in cell signaling.
- Glucose transport is vital for cellular energy production and proliferation.
Purpose of the Study:
- To investigate the mechanisms underlying early glucose transport activation in M07e cells stimulated by SCF and H2O2.
- To identify the specific signaling pathways involved in this activation process.
- To determine the role of glucose transporter 1 (GLUT1) in SCF- and H2O2-mediated glucose uptake.
Main Methods:
- Utilized M07e hematopoietic cell line.
- Measured glucose transport rates.
- Assessed GLUT1 protein levels in plasma membranes via translocation studies.
- Employed inhibitors of tyrosine kinases, phospholipase C (PLC), protein kinase C, phosphoinositide-3-kinase, and STI-571 (a c-kit inhibitor).
Main Results:
- Both SCF and H2O2 significantly increased Vmax for glucose transport.
- This enhancement was attributed to increased GLUT1 content in plasma membranes, likely via translocation from intracellular stores.
- Inhibitors of tyrosine kinases and PLC blocked glucose transport enhancement and GLUT1 translocation.
- STI-571 inhibited glucose transport activation by both SCF and H2O2, indicating a role for c-kit tyrosine kinase.
- Protein kinase C and phosphoinositide-3-kinase were not involved in the acute glucose transport activation.
Conclusions:
- SCF and H2O2 induce short-term glucose transport activation in M07e cells through GLUT1 translocation.
- Shared signaling pathways involving protein tyrosine kinases and PLC mediate glucose uptake activation by both stimuli.
- H2O2 may activate these pathways by increasing tyrosine phosphorylation, potentially through tyrosine phosphatase inhibition, mimicking endogenous ROS signaling.