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Updated: Aug 22, 2026

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
Published on: June 7, 2020
Oxysterol regulation of estrogen receptor alpha-mediated gene expression in a transcriptional activation assay system
Hiroyoshi Sato1, Satoko Nishida, Hiroko Tomoyori
1Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan. hiroyosi@junshin-c.ac.jp
Abstract:
In order to test the estrogenic activity of sterol oxidation products from cholesterol and phytosterols, an estrogen-dependent gene expression assay was performed in estrogen receptor alpha-stably transformed HeLa cells. The ranking of the estrogenic potency of these compounds was different: 17beta-estradiol >> genistein >> beta-epoxycholesterol = daidzein = cholestanetriol = 22(R)-hydroxycholesterol = 20(S)-hydroxycholesterol = sitostanetriol > campestanetriol = beta-epoxysitosterol = 7beta-hydroxycholesterol. These compounds were not estrogenic in estrogen receptor-negative HeLa cells.
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