Related Experiment Video
Updated: Aug 22, 2026

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Published on: December 5, 2019
Characterization of double-stranded RNA elements in the violet root rot fungus Helicobasidium mompa
Hideki Osaki1, Kinya Nomura, Naoyuki Matsumoto
1National Institute of Fruit Tree Science, Fujimoto, Tsukuba, Ibaraki 305-8605, Japan. hideki@affrc.go.jp
Abstract:
Double-stranded (ds) RNA of various types was detected by electrophoresis in 23 of 25 isolates of Helicobasidium mompa. These dsRNAs varied in size from ca. 2 kbp to more than 10 kbp. dsRNAs from an isolate V1 had two distinct nucleotide sequences for putative RNA-dependent RNA polymerase (RDRP). Their complete sequences revealed that V1 dsRNA1 was 2247 bp in length, with a single ORF that encoded a 706-amino acid residue polypeptide with a predicted molecular mass of 82.6 kDa, and that V1 dsRNA3 was 1776 bp in length, with a single ORF that encoded a 538-amino acid residue polypeptide with a predicted molecular mass of 62.6 kDa. RDRP-conserved motifs were identified in both predicted amino acid sequences. Phylogenetic analysis indicated that V1 dsRNA1 was most closely related to Fusarium poae virus 1, while V1 dsRNA3 was most closely related to Helicobasidium mompa 70 virus. These results indicate coinfection of isolate V1 by two distinct partitiviruses.
Related Concept Videos
Viruses with RNA Genomes
Subviral Agents
Viral Structure
Size and Structure of Viral Genomes
Viruses of Archaea

