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Updated: Jul 24, 2026
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
Published on: June 16, 2015
[Effect of exogenous collagen on cell function in construction of artificial biotendon in vitro]
1Department of Orthopedics, West China Hospital, Sichuan University, Chengdu Sichuan, PR China.
Objective:
To investigate the influence of the exogenous collagen on the function of cells in construction of artificial biotendon.
Methods:
Three materials including human hair, carbon fiber (CF) and polyglycolic acid (PGA) were combined with exogenous collagen and co-cultured with standard transferred human embryonic tenocytes at a concentration of 3 x 10(6)/mm3 in vitro. The cell number and morphology were observed under inverted microscope and scanning electron microscope after 2 hours, 3 days and 5 days.
Results:
In the artificial biotendon combined with collagen, the cells concentrated around the materials and the cells adhering to the materials turned into round after 2 hours. After 3 days, the adhering cells increased. After 5 days, the shape of the cells changed from round to spindle.
Conclusion:
Exogenous collagen will facilitate the cells to adhere onto materials and proliferate.
